![]() In conclusion, the two developed multiplex assays are suitable for quantification of GMO maize events and the same approach can be used in any other field with a need for accurate and reliable quantification of multiple DNA targets. ![]() This system seamlessly integrates a standard ddPCR workflow of droplet generation, thermal cycling, droplet reading, and analysis into a hands-free precision platform. As current analysis software does not support analysis of more than duplex, a new R- and Shiny-based web application analysis tool () was developed that automates the analysis of 4-plex results. The QX ONE ddPCR System is designed to deliver a precise and multiplexed digital PCR system. Per assay, two of the targets were labelled with one fluorophore and two with another. Two 4-plex assays were developed for quantification of 8 different DNA targets (7 genetically modified maize events and maize endogene). Bio-Rad partners with test kit providers to develop Droplet Digital PCR (ddPCR). The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR. Now the QX600 System provides advanced, 6-color multiplexing to maximize the number of biomarkers interrogated per well, allowing the preservation of precious samples and reducing lab burden. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. Bio-Rad Laboratories 64.9K subscribers Subscribe 24K views 6 months ago Droplet Digital PCR (ddPCR) This video showcases the QX600 Droplet Digital PCR System which enables advanced. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. The QX600 Droplet Digital PCR System requires the use of the C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.The advantages of the digital PCR technology are already well documented until now. Wastewater testing - Leverage the QX600 System to perform population-wide monitoring of established and emerging pathogens.Food testing - Use the QX600 System to perform routine evaluation of genetically modified organisms (GMO) and pathogen contamination detection.Bruker launched the Canopy CellScape, its latest ChipCytometry platform for spatial biology. The relatively compact system features a fully integrated droplet generator and four color reader, saving bench space. Cell and gene therapy - Accelerate therapeutics discovery with highly-advanced multiplexing afforded by the QX600 System. Bio-Rad also displayed the QX1 dPCR platform, which has been on the market for a year.Next generation sequencing (NGS) orthogonal testing - With the QX600 System perform accurate quantification and qualification of NGS libraries without the use of a standard curves.Gene Expression - Employ the extreme precision of the QX600 System to quantify small-fold changes in low abundance RNA molecules. The QX600 System is designed for researchers who need to quantify multiple targets with high accuracy, reproducibility, and sensitivity.Oncology research - The QX600 System collects more information per well, preserving precious circulating tumor DNA (ctDNA) and tumor samples.The QX600 System enhances a wide variety of applications, including: oncology research, gene expression, next generation sequencing (NGS) orthogonal testing, cell and gene therapy, food and wastewater testing. System capable of running both TaqMan® and EvaGreen chemistriesĪpplications of the QX600 Droplet Digital PCR System.Extensive menu of pre-existing ddPCR assays.Cross-compatibility with QX200 reagents, assays, and consumables. ![]()
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